THE IMPACT OF TROFIC EFFECTS OF MULTI STRAIN PROBIOTIC PREPARATION ON PARACELLULAR PERMEABILITY
The article “The impact of trofic effects of multi strain probiotic preparation on paracellular permeability” reports the most relevant scientific results from a study conducted by two reserachers team from Catholic University Of Sacred Heart Of Rome and of A. Gemelli Hospital (V. Petito, L. Laterza, C. Graziani, L. Lopetuso, F. Scaldaferri, G. Gasbarrini, A. Gasbarrini, D. Lucchetti, A. Sgambato).
The aim of the study was to evaluate the mechanism of action of VSL#3 (multistrain probiotic) to reduce intestinal permeability, and its effect in modulating the activity on tight junctions, focusing on the effects of secreted bioactive factors.
The multistrain probiotic formula, in particular the more concentrate CM (1:10) increase Caco2 and HT29 cells proliferation and the percentage of metabolic cell activity (up 20%), together with a decrease of 90% of apoptotic cells in the presence of CM. These trophic effects may have an impact on paracellular permeability. The expression of tight junction proteins increase in 24 h for claudin and ZO-1, and after 48 h of treatment in case of occludin. Also the densitometry and the ratio between of relative density of protein and endogenous control (β-actin) confirms these data. The results are the same using two different lots.
These preliminary data in vitro could be able to explain how VSL#3 works at intestinal mucosa level, in particular by secretion of factors that enhances barrier integrity. The proliferative stimuli and the increase of expression of tight junction proteins are consistent with an effect on mucosa regeneration and re-epithelization.